Position

Associate Professor
College of Medicine and Public Health

Biography

I completed my undergraduate degree then worked in Plant Virology with the late Prof Bob Symons before completing my PhD with Prof John Wallace, University of Adelaide. I then worked as a post-doc with Professor Chris Burrell and Dr Peng Li at the IMVS/SA Pathology from 1996-2008, part-time from 1998-2011 due to family commitments, studying the basic mechanisms of replication of HIV and virus-host interactions. From 2008-2011 I was also involved in the diagnostic management of clinical HIV drug resistance. At the same time I developed my own research interests in dengue virus stemming from an initial collaboration with A/Professor Peter Wright (Monash University). In March 2011 I re-located to my current full-time position at Flinders University. My current research interests continue in Medical Virology, in particular dengue virus and the interaction with the host cell that is associated with pathogenesis.

Qualifications

  • BSc, University of Adelaide, 1990
  • BSc, First Class Hons, University of Adelaide, Department of Biochemistry, 1991
  • PhD, Department of Biochemistry, University of Adelaide, 1995

Honours, awards and grants

For a full publication list go to Google Scholar https://scholar.google.com.au/citations?user=HdsCukAAAAAJ&hl=en

Key responsibilities

Research Activities: Laboratory head
Teaching: Microbiology and Infectious Diseases in first year graduate entry Medicine, second and third year Bachelor of Medical Science

Teaching interests

Medical microbiology and virology, in particular Dengue virus.

For more information: studying virology at Flinders University.

BMedSci year 2 course co-ordinator

Topic Coordinator:

  • MMED2935  Human Immunology and Infectious Diseases
  • MMED3939  Virology, Immunology and Immunopathogenesis

Topic Lecturer:

  • MMED8120A Knowledge of Health and Illness 1A - IMD

Research expertise

  • Medical microbiology

Research interests

Overall theme of host responses to viral infections and determinants of pathogenesis. For more information about the lab, go to Virology Research.

  1. DENV regulation of sphingosine kinase 1 (SK1) activity. In collaboration with Prof Stuart Pitson, Centre for Cancer Biology, SA Pathology. We have shown that DENV infection changes the cellular activity of an SK1, an important lipid kinase that regulates intracellular signalling and the cell survival/death decision. Current work centres on understanding how DENV influences SK1 activity and the consequences for DENV replication and changes in host cell function.
  2. DENV effects on the endothelium. In collaboration with Dr Claudine Bonder, Centre for Cancer Biology, SA Pathology. An important cell target for the pathogeneic effects of DENV is the endothelium. We will investigate parameters effecting DENV infection of the endothelium, the cellular response to infection and the effect of this on endothelial function.
  3. The innate immune response and DENV. In collaboration with A/Prof Michael Beard and Dr Karla Helbig, School of Molecular and Biomedical Science, University of Adelaide. We have shown that the interferon stimulated gene, viperin, is up-regulated in DENV-infected cells and acts to inhibit DENV replication. Investigation of innate responses and ISG's in the context of (1) and (2) above is of interest. Addtionally, we more recently have begun to investigate the interatction between DENV and factor H, a negative regulator of the alternative complement pathway in collaboration with Professor Dave Gordon, Head of MID at SOM, Flinders University.

Supervisory interests

  • Dengue virus
  • Virus, virology

RHD research supervision

Current

Principal supervisor : Analysis of the role of sphingosine kinase in dengue virus replication and pathogenesis (1) ; Microbiology and infectious diseases (1) ; Role of sphinhisane kinase and endotherlial cells in dengue virus pathogenesis. (1) ;

Completion

Principal supervisor : Altered responses of dengue virus infected cells to TNF-a (Univ of Adelaide) (1) ;

Publications

  • Al Shujairi, W.H., Clarke, J.N., Davies, L., Alsharifi, M., Pitson, S. and Carr, J.M. (2017). Intracranial injection of Dengue Virus induces interferon stimulated genes and CD8+ T cell infiltration by Sphingosine Kinase 1 independent pathways. PLoS One, 12(1) pp. Art: e0169814.
    [10.1371/journal.pone.0169814]
  • Aloia, A.L., Calvert, J.K., Clarke, J.N., Davies, L., Helbig, K., Pitson, S.M., et al. (2017). Investigation of sphingosine kinase 1 in interferon responses during dengue virus infection. Clinical & Translational Immunology, 6 pp. Art: e151.
    [10.1038/cti.2017.32]
  • Carr, J.M., Ashander, L.M., Calvert, J.K., Ma, Y., Aloia, A.L., Bracho Granado, G.R., et al. (2017). Molecular responses of human retinal cells to infection with Dengue Virus. Mediators of Inflammation,
  • Van Der Hoek, K., Eyre, N.S., Shue, B., Khantisitthiporn, O., Glab-Ampi, K., Carr, J.M., et al. (2017). Viperin is an important host restriction factor in control of Zika virus infection. Nature, 7(1) pp. Art: 4475.
    [10.1038/s41598-017-04138-1]
  • Ali, M.E., Rajapaksha, K.H., Carr, J.M. and Petrovsky, N. (2016). Norovirus drug candidates that inhibit viral capsid attachment to human histo-blood group antigens. ANTIVIRAL RESEARCH, 133 pp. 14-22.
    [10.1016/j.antiviral.2016.07.006] [Scopus]
  • Clarke, J.N., Davies, L.K., Calvert, J.K., Gliddon, B.L., Al Shujari, W.H., Aloia, A.L., et al. (2016). Reduction in sphingosine kinase 1 influences the susceptibility to dengue virus infection by altering antiviral responses. Journal of General Virology, 97(1) pp. 95-109.
    [10.1099/jgv.0.000334] [Scopus]
  • Senaratne, T., Carr, J.M. and Noordeen, F. (2016). Elevation in liver enzymes is associated with increased IL-2 and predicts severe outcomes in clinically apparent dengue virus infection. Cytokine, 83 pp. 182-188.
    [10.1016/j.cyto.2016.04.010] [Scopus]
  • Senaratne, T., Wimalaratne, H., Alahakoon, D.G., Gunawardane, N., Carr, J.M. and Noordeen, F. (2016). Characterization of dengue virus infections in a sample of patients suggests unique clinical, immunological and virological profiles that impact on the diagnosis of dengue and dengue hemorrhagic fever. Journal of Medical Virology,
    [10.1002/jmv.24525] [Scopus]
  • Aloia, A.L., Abraham, A.M., Bonder, C., Pitson, S. and Carr, J.M. (2015). Dengue Virus-induced inflamamtion of the endothelium and the potential roles of Sphingosine Kinase-1 and MicroRNAs. Mediators of Inflammation, 2015(Art: 509306)
    [10.1155/2015/509306]
  • Milic, N.L., Davis, S.B., Carr, J.M., Isberg, S., Beard, M.R. and Helbig, K. (2015). Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus) Developmental and Comparative Immunology, 51 pp. 108-115.
    [Scopus]
  • Calvert, J.K., Helbig, K., Dimasi, D.P., Cockshell, M.P., Beard, M.R., Pitson, S., et al. (2015). Dengue Virus infection of primary endothelial cells induces innate immune responses, changes in endothelial cells function and is restricted by Interferon-stimulated responses. Journal of Interferon and Cytokine Research, 35(8) pp. 654-665.
    [10.1089/jir.2014.0195] [Scopus]
  • Aloia, A., Eyre, N., Black, S., Bent, S., Gaeguta, A., Guo, Z., et al. (2014). Generation of a chimeric hepatitis C replicon encoding a genotype-6a NS3 protease and assessment of boceprevir (SCH503034) sensitivity and drug-associated mutations. Antiviral Therapy, Sept 15th
    [10.3851/IMP2850] [Scopus]
  • Boon, V.A.F., Carr, J. and Klebe, S. (2013). The role of viruses in carcinogenesis. Australian Medical Student Journal, 4(2) pp. 11-15.
    [Web Link]
  • Carr, J., Kua, T., Clarke, J., Calvert, J., Zebol, J., Beard, M., et al. (2013). Reduced sphingosine kinase 1 activity in dengue virus type-2 infected cells can be mediated by the 3' untranslated region of dengue virus type-2 RNA. Journal of General Virology, 94(PART 11) pp. 2437-2448.
    [10.1099/vir.0.055616-0] [Scopus]
  • Helbig, K., Carr, J., Calvert, J., Wati, S., Clarke, J., Eyre, N.S., et al. (2013). Viperin is induced following Dengue Virus Type-2 (DENV-2) infection and has anti-viral actions requiring the C-terminal end of viperin. Plos Neglected Tropical Diseases, 7(4) pp. e2178.
    [10.1371/journal.pntd.0002178] [Scopus]
  • Carr, J., Mahalingam, S., Bonder, C. and Pitson, S. (2013). Sphingosine kinase 1 in viral infections. Reviews in Medical Virology, 23(2) pp. 73-84.
    [10.1002/rmv.1718] [Scopus]
  • Wati, S., Rawlinson, S., Ivanov, R., Dorstyn, L., Beard, M., Jans, D., et al. (2011). Tumour necrosis factor alpha (TNF-alpha) stimulation of cells with established dengue virus type 2 infection induces cell death that is accompanied by reduced ability of TNF-alpha to activate nuclear factor kB and reduced sphingosine kinase1 activity. Journal of General Virology, 92(4) pp. 807-818.
    [10.1099/vir.0.028159-0] [Scopus]
  • Carr, J., Green, T., Shaw, D., Daly, L., Hart, W., Ratcliff, R., et al. (2009). Application of an allele specific PCR to clinical HIV genotyping samples detects additional K103N mutations in both therapy naïve and experienced patients. Journal of Medical Virology, 81(12) pp. 1983-1990.
    [10.1002/jmv.21628] [Scopus]
  • Wati, S., Soo, M.L., Zilm, P., Li, P., Paton, A., Burrell, C., et al. (2009). Dengue Virus Infection Induces Upregulation of GRP78, Which Acts To Chaperone Viral Antigen Production. Journal of Virology, 83(24) pp. 12871-12880.
    [10.1128/JVI.01419-09] [Scopus]
  • Davis, A., Carr, J., Bagley, C., Powell, J., Warrilow, D., Harrich, D., et al. (2008). Human immunodeficiency virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells. Retrovirology, 5
    [10.1186/1742-4690-5-115] [Scopus]
  • Carr, J., Coolen, C., Davis, A., Burrell, C. and Li, P. (2008). Human immunodeficiency virus 1 (HIV-1) virion infectivity factor (Vif) is part of reverse transcription complexes and acts as an accessory factor for reverse transcription. Virology, 372(1) pp. 147-156.
    [10.1016/j.virol.2007.10.041] [Scopus]
  • Carr, J., Cheney, K., Coolen, C., Davis, A., Shaw, D., Ferguson, W., et al. (2007). Development of methods for co-ordinate measurement of total cell associated and integrated HIV-1 DNA forms in routine clinical samples: levels are not associated with clinical parameters but low levels of integrated HIV-1 DNA may be prognostic for co. Journal of Clinical Microbiology, 45(4) pp. 1288-1297.
    [10.1128/JCM.01926-06] [Scopus]
  • Wati, S., Li, P., Burrell, C. and Carr, J. (2007). Dengue virus (DV) replication in monocyte-derived macrophages is not affected by tumor necrosis factor alpha (TNF-A) and DV infection induces altered responsiveness to TNF-A stimulation. Journal of Virology, 81(18) pp. 10161-10171.
    [10.1128/JVI.00313-07] [Scopus]
  • Carr, J., Davis, A., Feng, F., Burrell, C. and Li, P. (2006). Cellular interactions of virion infectivity factor (Vif) as potential therapeutic targets: APOBEC3G and more? Current Drug Targets, 7(12) pp. 1583-1593.
    [10.2174/138945006779025356] [Scopus]
  • Carr, J., Davis, A., Coolen, C., Cheney, K., Burrell, C. and Li, P. (2006). Vif-deficient HIV reverse transcription complexes (RTCs) are subject to APOBEC3G-mediated structural changes and mutation of RTC associated reverse transcription products. Virology, 351(1) pp. 80-91.
    [10.1016/j.virol.2006.03.027] [Scopus]
  • Arriaga, M., Carr, J., Li, P., Wang, B. and Saksena, N. (2006). Interaction between HIV-1 and APOBEC3 sub-family of proteins. Current HIV research, 4(4) pp. 401-409.
    [10.2174/157016206778560063] [Scopus]
  • Davis, A., Cheney, K., Carr, J., Karlis, J., Vandegraaff, N., Coolen, C., et al. (2006). Measurement of integrated HIV-1 DNA in patient samples and infected cells treated with candidate integration inhibitors. Current Topics in Virology, 5 pp. 9-19.
    [Web Link]
  • Feng, F., Davis, A., Lake, J.A., Carr, J., Xia, W., Burrell, C., et al. (2004). Ring finger protein ZIN interacts with Human Immunodeficiency Virus Type 1 Vif. Journal of Virology, 19 pp. 10574-10581.
    [10.1128/JVI.78.19.10574-10581.2004] [Scopus]
  • Carr, J., Hocking, H., Bunting, K., Wright, P., Davidson, A., Gamble, J., et al. (2003). Supernatants from dengue virus type-2 infected macrophages induce permeability changes in endothelial cell monolayers. Journal of Medical Virology, 69(4) pp. 521-528.
    [10.1002/jmv.10340] [Scopus]
  • Lake, J.A., Carr, J., Feng, F., Mundy, L., Burrell, C. and Li, P. (2003). The role of Vif during HIV-1 infection: interaction with novel host cellular factors. Journal of Clinical Virology, 26 pp. 143-152.
    [Scopus]
  • Carr, J., Calvert, J., Kumar, R., Burrell, C. and Li, P. (2001). Effect of deletion and the site of insertion in double copy anti-tat retroviral vectors : viral titres and production of anti-tat mRNA. Archives of Virology, 146(11) pp. 2191-2200.
    [10.1007/s007050170029] [Scopus]
  • Pryor, M., Carr, J., Hocking, H., Davidson, A., Li, P. and Wright, P. (2001). Replication of dengue virus type 2 in human monocyte-derived macrophages: comparison of isolates and recombinant viruses with substitutions at amino acid 390 in the envelope glycoprotein. American Journal of Tropical Medicine and Hygiene, 65(5) pp. 427-434.
    [Scopus] [Web Link]
  • Carr, J., Hocking, H., Li, P. and Burrell, C. (1999). Rapid and efficient cell-to-cell transmission of human immunodeficiency virus infection from monocyte-derived macrophages to peripheral blood lymphocytes. Virology, 265(2) pp. 319-329.
    [10.1006/viro.1999.0047] [Scopus]
  • Carr, J., Ramshaw, H., Li, P. and Burrell, C. (1998). CD34+ cells and their derivatives contain mRNA for CD4 and human immunodeficiency virus (HIV) co-receptors and are susceptible to infection with M- and T-tropic HIV. Journal of General Virology, 79(1) pp. 71-75.
    [Scopus] [Web Link]
  • Lucic, M., Forbes, B.E., Grosvenor, S., Carr, J., Wallace, J. and Forsberg, G. (1998). Secretion in Escherichia coli and phage-display of recombinant insulin-like growth factor binding protein-2. Journal of Biotechnology, 61 pp. 95-108.
    [Scopus]
  • Al Shujairi, W.H., Clarke, J.N., Davies, L., Alsharifi, M., Pitson, S. and Carr, J.M. (2017). Intracranial injection of Dengue Virus induces interferon stimulated genes and CD8+ T cell infiltration by Sphingosine Kinase 1 independent pathways. PLoS One, 12(1) pp. Art: e0169814.
    [10.1371/journal.pone.0169814]
  • Aloia, A.L., Calvert, J.K., Clarke, J.N., Davies, L., Helbig, K., Pitson, S.M., et al. (2017). Investigation of sphingosine kinase 1 in interferon responses during dengue virus infection. Clinical & Translational Immunology, 6 pp. Art: e151.
    [10.1038/cti.2017.32]
  • Carr, J.M., Ashander, L.M., Calvert, J.K., Ma, Y., Aloia, A.L., Bracho Granado, G.R., et al. (2017). Molecular responses of human retinal cells to infection with Dengue Virus. Mediators of Inflammation,
  • Van Der Hoek, K., Eyre, N.S., Shue, B., Khantisitthiporn, O., Glab-Ampi, K., Carr, J.M., et al. (2017). Viperin is an important host restriction factor in control of Zika virus infection. Nature, 7(1) pp. Art: 4475.
    [10.1038/s41598-017-04138-1]
  • Ali, M.E., Rajapaksha, K.H., Carr, J.M. and Petrovsky, N. (2016). Norovirus drug candidates that inhibit viral capsid attachment to human histo-blood group antigens. ANTIVIRAL RESEARCH, 133 pp. 14-22.
    [10.1016/j.antiviral.2016.07.006] [Scopus]
  • Senaratne, T., Wimalaratne, H., Alahakoon, D.G., Gunawardane, N., Carr, J.M. and Noordeen, F. (2016). Characterization of dengue virus infections in a sample of patients suggests unique clinical, immunological and virological profiles that impact on the diagnosis of dengue and dengue hemorrhagic fever. Journal of Medical Virology,
    [10.1002/jmv.24525] [Scopus]
  • Clarke, J.N., Davies, L.K., Calvert, J.K., Gliddon, B.L., Al Shujari, W.H., Aloia, A.L., et al. (2016). Reduction in sphingosine kinase 1 influences the susceptibility to dengue virus infection by altering antiviral responses. Journal of General Virology, 97(1) pp. 95-109.
    [10.1099/jgv.0.000334] [Scopus]
  • Senaratne, T., Carr, J.M. and Noordeen, F. (2016). Elevation in liver enzymes is associated with increased IL-2 and predicts severe outcomes in clinically apparent dengue virus infection. Cytokine, 83 pp. 182-188.
    [10.1016/j.cyto.2016.04.010] [Scopus]
  • Aloia, A.L., Abraham, A.M., Bonder, C., Pitson, S. and Carr, J.M. (2015). Dengue Virus-induced inflamamtion of the endothelium and the potential roles of Sphingosine Kinase-1 and MicroRNAs. Mediators of Inflammation, 2015(Art: 509306)
    [10.1155/2015/509306]
  • Milic, N.L., Davis, S.B., Carr, J.M., Isberg, S., Beard, M.R. and Helbig, K. (2015). Sequence analysis and characterisation of virally induced viperin in the saltwater crocodile (Crocodylus porosus) Developmental and Comparative Immunology, 51 pp. 108-115.
    [Scopus]

Professional and community engagement

Local Organising committee for the Asia Pacific Congress of Medical Virology 2012

Local Organising committee for the Australian Society for Microbiology Conference, Adelaide, 2013

Committee Member, Australasian Society for Virology (AVS) 2013-current

Reviewer, Flinders University Institutional Biosafety Committee (IBC), 2012-current

Contact

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Phone: +61 8 82046658
Email:
Location: Flinders Medical Centre (5D:316)
Postal address: GPO Box 2100, Adelaide 5001, South Australia
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