Performs assays for characterising the properties of particles - usually cells. Functional assays include measurement of Calcium flux, apoptosis and phenotype based on cell membrane or cytoplasmic antigen expression. Flow cytometry can also be used for DNA analysis and cell cycle analysis. There is an extensive range of assays available. The Flow Cytometry Facility is equipped with state of the art flow cytometers used for:

  • Cell identification and analysis
  • Cell sorting
  • Cell function examination
  • Measurement of cellular membrane electric potential
  • DNA analysis
  • Diagnosis of diseases such as Leukemia and Lymphoma

The Facility is based at FMC and has a diagnostic role in the FMC hospital as part of SouthPath. Dr Peter J. Macardle runs the laboratory and has over thirty years flow cytometry experience. Dr. Sheree Bailey is the Senior Scientist and is one of the most experienced cytometrists in Australia. This facility provides a full range of diagnostic tests covering the following areas:

  • Autoimmunity
  • Allergy
  • Immunoassay
  • Protein Electrophoresis
  • Cellular Immunology
  • Renal and Skin Biopsies
  • Immunodeficiency

Instrumentation

  • BD FACS Canto, equipped with a 633nm and a 488nm laser, this machine is capable of eight parameter analysis comprising two physical parameters and six fluorescent parameters.
  • BD FACS Aria, equipped with a 488nm, 633nm and 405nm laser. This machine is a high-speed cell sorter capable of sorting at up to 25,000 cells per second. Its fluorescent capability is up 13 fluorescent parameters and two physical parameters.
  • BD FACScan, this is a three fluorescent parameter and two physical parameter analytical machine it is equipped with one 488nm laser. Beckman Coulter Biomek 3000 robotic liquid handler
  • BD FACS Vantage SE, this is a cell sorter equipped with a 325nm, 633nm and 488nm laser. This is 5-6 fluorescent machine with a capability of using true UV excitation.