Proteomics

Provides a broad range of standard and advanced research techniques for the analysis of proteins in a broad range of research fields including neuroscience, biotechnology, cancer, ophthalmology, intracellular signalling and immunology. The advanced capabilities of the facility attract researchers from major research institutions across Australia.

Services Currently Available:

• Gel Electrophoresis:
  • 1D Gels
  • Mini gel Western Blots
  • 2D Gels and clean up kits
  • EZQ protein Quantification
  • DIGE analysis
• Stains:
  • Silver Mini gel and large format gels
  • Sypro Ruby
  • Pro Q Diamond Phosphorylation
  • Pro Q Emerald Glycosylation
• Robot:
  • Trypsin Digest
  • ELISA
  • Protein assay
  • Liquid handling
• Others:
  • ProteomeLabo

Instrumentation:

• Dionex Ultimate 3000 nanoflow HPLC
• Thermo LTQ XL linear ion trap mass spectrometer
• Beckman Coulter ProteomeLab PF2D 2D HPLC
• Beckman Coulter Biomek 3000 robotic liquid handler
• GE Akta FPLC protein purification system \
• GE IPGPhor3 isoelectric focusing units
• GE Ettan 2D electrophoresis equipment
• GE Typhoon Fluorescence Imager
• GE VDS-CL CCD imager