Flinders Genomics Facility

 

Flinders Genomic Facility

 

 

  Welcome

The Flinders Genomics Facility is a specialised environment for conducting high-throughput genetic and genomic research. Our experienced staff utilise a variety of state-of-the-art platforms to generate high-quality genomic data and to support its analysis.

We provide genomics services for researchers across Flinders University, including biotechnology, pathology, biomedical and biological research clients, as well as groups at other academic or commercial institutions. Please contact us to discuss how we can assist you in your research.

Our mission is to facilitate scientific discovery and to develop innovative approaches that can extend the application and scientific relevance of genomic analysis. We use established but powerful technologies for next-generation sequencing and high-throughput genotyping.

Affordable pricing

Queries about sequencing and screening projects of any size, including small-scale experiments to test new approaches are welcomed. Our goal is to make next-generation sequencing and high-throughput screening accessible to as many researchers as possible. By doing so, we not only advance the use of these experimental techniques, but also facilitate the kinds of discoveries that are only possible using high-throughput technologies.


 

What we offer:

  • Full library preparation service
  • Rapid sample turnaround
  • Sequencing service on the Illumina and Ion Torrent platforms
  • Lab Chip service
  • Pippin Prep service
  • Covaris service
  • E-Gel use

 

Our facility

Facility Manager: Dr Renee Smith, PhD

Assistant Manager: Ms Letitia Pimlott

Bioinformatics: Dr Shashikanth Marri, PhD

Location: Flinders Centre for Innovation in Cancer (FCIC), Rm 4L160

Email: genomicsfacility@flinders.edu.au

Phone 8204 5446 or ext. 65446

 

FLINDERS GENOMICS FACILITY

Flinders Centre for Innovation in Cancer, Room 4L160
genomicsfacility@flinders.edu.au
Ext: 65446 or 8204 5446

MiSeq System – illumina

MiSeq sequencing by synthesis chemistry is the most widely adopted next-generation sequencing technology. Data quality is  achieved by a proprietary, reversible terminator-based method that detects single bases as they are incorporated into massively parallel DNA strands. The MiSeq generates up to 25 million, 2 x 300 bp paired end reads, or 10 Gb of sequence data.

NextSeq - illumina

The NextSeq 500 desktop sequencing system provides     the flexible power and simplicity you need to analyse whole genomes, exomes, and transcriptomes. The NextSeq generates up to 400 million reads, 2x150 bp paired end reads, or 120 Gb of sequence data.


HiSeq - illumina

The HiSeq 2500 System is a powerful and efficient ultra-high-throughput sequencing system that supports the broadest range of applications and study sizes. The HiSeq system generates up to 3 billion reads.

Ion Proton System – Life Technologies

 Ion Torrent uses semiconductor sequencing technology to deliver the fastest bench top next generation sequencing. This technology offers 200bp read lengths.

 

            Ion P1 Chip: 10 Gb of sequence data

           

 

Ion Personal Genome Machine (PGM) System – Life Technologies

Ion Torrent uses semiconductor sequencing technology to deliver the fastest bench top next generation sequencing. This technology offers 200 bp or 400 bp read lengths and can be sequenced on 3 different Ion Chips.

Ion 314 Chip: 30 – 100 Mb of sequence data
Ion 316 Chip: 300 Mb – 1 Gb of sequence data
Ion 318 Chip: 600 Mb – 2 Gb of sequence data

LabChip GX Touch 24 Capillary Electrophoresis – Perkin Elmer

The Lab Chip utilises microfluidic technology to qualitatively and quantitatively analyse nucleic acid samples.

 

Pippin Prep – Sage Science

The Pippin Prep is a preparative electrophoresis platform that separates and extracts DNA fragments using pre-cast and disposable gel cassettes. The DNA is automatically collected in buffer according to software-input size ranges. Each pre-cast gel contains 5 lanes.

 Image result for covaris

Covaris M220 - Covaris

The Covaris is a DNA/RNA shearing method that uses Adaptive Focused Acoustics (AFA) technology under isothermal conditions to maintain the integrity of the nucleic acid sample. Mechanical shearing with AFA technology is the gold standard for nucleic acid fragmentation during DNA library preperation.