Flow cytometry is now commonly used in both research and clinical laboratories to identify and quantify particles, usually cells, based on their physical or molecular characteristics and/or expression of specific proteins.
Assays that can be performed by flow cytometry include measurement of calcium flux, apoptosis, changes in protein expression in the cell membrane or cytoplasm and assessment of DNA quantity and integrity for determining cell cycle distribution. Flow cytometry also represents a means of detecting viruses, bacteria and plankton in water samples.
The Flow Cytometers in the Flinders University facility are equipped to :
- Identify and characterise a wide range of cell types
- Sort and purify cell fractions
- Assess cell functions
- Measure mitochondrial membrane potential
- Analyse DNA
- Measure cell death (apoptotic and necrotic)
- Analyse small particles, e.g. exosomes
With the state-of-the art Image Stream-X instrument in the facility, which combines the power of microscopy with flow cytometry, it is now also possible to perform a rapid and highly detailed examination of a large number of cells in each sample.
The fee-for-service facility is based at Flinders Medical Centre (FMC). We offer flow cytometry training, assistance and advice to staff and students in regards to the implementation of flow cytometry into on-going or new research projects.