South Australian Genomics Centre (SAGC) - Flinders Node

The SAGC offers both Illumina and MGI sequencing. We offer a range of next generation sequencing platforms to find an efficient match for projects of any size. See our range of next generation sequencers with available flow cells and output.

STOmics (SpaTial Enhanced Resolution Omics-Sequencing): whole transcriptomics for discovery applications (range of 1000-2000 genes/cell) leverages in situ mRNA capture and cDNA sequencing with barcoded spatial coordinates to create a spatial transcriptomic profile of the tissue section for analysis.

• Subcellular spatial localization of mRNA
• Chip design ~320M spatially barcoded spots/cm2
• Spot size = 200 nm diameter
• Range of field size to accommodate larger tissue samples (up to 13 cm x 13cm)

Xenium Analyser allows targeted gene expression and protein quantification at subcellular resolution. Xenium is an end-to-end platform that enables high-throughput subcellular mapping of RNA targets.

• Menu of off-the-shelf gene panels + order 100 custom add-on genes
• Compatible with fresh frozen (FF) and formalin-fixed, paraffin-embedded (FFPE) tissues
• Microscopy based read-out (no NGS), allows H&E post-run
• Single molecule RNA and Protein targets (subcellular resolution)

VisiumHD Spatial Transcriptomics allows whole transcriptome mapping within morphological context of complex tissue samples. There is no need to select targets or specific region of interest. Compatible with FFPE and is CytAssist enabled which allows transfer of archived tissue slides to the special barcoded Visium slides to both improve workflow and reduce RNA diffusion.

Genomics SAGC has a Chromium Controller and Chromium X Series from 10X Genomics to enable multiomic analysis through droplet GEM-based single cell partitioning and sequencing.

Applications available:

• Single cell RNA expression
• Single cell Immune Profiling
• Single cell ATAC
• Single cell ATAC + RNA expression
• Single cell RNA expression Flex (FFPE)

*Cell surface marker tagging can be incorporated into some of these techniques and quantified during sequencing.

Compatible with fixed, fresh, and frozen samples, as well as whole cells and nuclei.

Evercode is a plate-based combinatorial barcoding technology that enables very large single cell experiments (up to 1 M cells or 96 samples) in a single workflow with either fixed or fresh cells. This approach can offer some advantages with certain cell types and achieve higher throughput at a lower cost compared to microfluidic based approaches.