Leica SP5 Confocal Microscope
The Leica SP5 laser scanning confocal microscope features tunable emission range detection which can be adjusted to any bandwidth in the visible to near far-red spectra as well as Fluorescence Correlation Spectroscopy (FCS) capability. It is equipped with 405, 458, 488, 496, 514, 561 and 633 nm lasers lines, five PMT detectors, a transmitted light detector with DIC optics, and two single photon sensitive filtercube-based detectors (avalanche photo-diode detectors) and a resonant scanner (8kHz) for high sensitivity or high speed scanning, respectively. This inverted microscope is equipped with a range of water, glycerol and oil immersion lenses and is suitable for fixed sample imaging and short term live cell imaging with temperature (but not CO2 or humidity) control. A variety of high-end techniques such as FRET-AB, FRET-SE, FRAP are made easier with software wizards.
In person training to be completed with relevant staff.
Dr Jen Fendler